Brucella is a genus of Gram-negative bacteria, named after David Bruce (1855–1931). They are small (0.5 to 0.7 by 0.6 to 1.5 µm), nonencapsulated, nonmotile, facultatively intracellular coccobacilli.
Brucella spp. are the cause of brucellosis, which is a zoonosis transmitted by ingesting contaminated food (such as unpasteurized milk products), direct contact with an infected animal, or inhalation of aerosols. Transmission from human to human, for example through sexual intercourse or from mother to child, is exceedingly rare, but possible. Minimum infectious exposure is between 10 and 100 organisms.
1) Binding Rate formula: (Sample O.D.÷Negative Control O.D.)×100%
2) The animal source is healthy: Binding rate of the samples is ≥50%
3) The animal source is under infection: Binding rate of the samples is < 40%
4) The animal source is suspected of being infected: Binding rate is 40%~50%
1) Sensitivity: 100%
2) Specificity: there is no cross-reaction with other substances in the samples.
3) Security: Pre-coated antigen, HRP-conjugated antibodies and controls only have immunogenicity, non-microorganism toxicity and infectivity.
4) Intra-assay: CV<10% (n=3).
5) Inter-assay: CV<15% (n=9).
6) The experimental results should meet the following conditions. Should any of these criteria not be fulfilled, the test is invalid and the assay should be repeated. : Mean OD of Negative control is OD450nm＞0.50.
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