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1.   Protocols for ELISA

 1)     Direct ELISA
 2)     Direct ELISA Using Fluorescent Substrate
 3)     Indirect ELISA
 4)     Sandwich ELISA

2.   Protocols for IHC ICC

 1)     Determining if the antibody binds only phosphorylated protein (WB or IHC)

 2)     Double immunofluorescence-sequential protocol

 3)     Double immunofluorescence-simultaneous protocol

 4)     Fixation and Permeabilization In IHC ICC

 5)     Glycol Methalacrylate Acrylic Resin Embedding For IHC

 6)     IHC Troubleshooting Tips

 7)     IHC-Paraffin (IHC-P)

 8)     Immunocytochemistry (ICC)

 9)     Immunohistochemistry (IHC-Fr) - Frozen Sections

 10)   Mouse-on-Mouse Staining

 11)   Perfusion Fixation

3.   Protocols for WB

 1)     Histone Extraction

 2)     Mitochondrial Purification

 3)     Nuclear Fractionation

 4)     S-100 Mitochondrial Fractionation

 5)     Stripping for Reprobing Western Blots

 6)     WB Troubleshooting Tips

 7)     Western Blotting - A Beginner's Guide

 8)     Western Blotting of Phospho-Proteins

 9)     Western Blotting Using Antibodies Against Histone Proteins

4.   Protocols for IP

 1)     Immunoprecipitation (IP)

 2)     Using IgM antibodies for IP

5.   Protocols for FACS

 1)     Direct Staining Protocol (Cell Surface Staining)

 2)     FACS Troubleshooting Tips

 3)     Flow Cytometry Whole Blood Samples-Red Blood Cell Lysis

 4)     Indirect Staining Protocol (Cell Surface Staining)

 5)     Intracellular Staining

 6)     Recommended Controls for FACS

6.   Protocols for ELISPOT

 1)     ELISPOT

 2)     ELISPOT Troubleshooting Tips