Three novel single-nucleotide polymorphisms of complement component 4 gene (C4A) in Chinese Holstein cattle and their associations with milk performance traits and CH50
2012-01-201473
abstract
Complement component 4 (C4A) is a candidate gene that reflects complement activity. Theprimary role of this gene in the classical and lectin-activation pathways is to provide protectionagainst bacterial pathogens. In the current study, the bovine complement C4A genewas screened for polymorphisms, and the associations of these polymorphisms with thehemolytic activity of the classical pathway (CH50), C4 serum levels, and milk performancetraits were examined. Three novel single-nucleotide polymorphisms (rs 132741478: g.2994A > G, rs 134006517: g.3508 A > G, and rs 137485678: g.3649 G > C) were detected by DNAsequencing and PCR-RFLP in 1182 Chinese Holstein cows. The rs132741478: g.2994 A > Gmutation in exon 10 led to methionine and valine exchange at position 362, whereas rs134006517: g.3508 A >Gandrs137485678: g.3649 G > C were synonymous substitutions.The statistical analyses revealed that cows with rs 132741478: g.2994 A>GAGandrs137485678: g.3649 G > C-CC have significantly lower somatic cell scores (SCS, P < 0.01).Homozygote cows with GAC haplotypes have the lowest SCS, whereas AAG/AAC cows havethe highest. The serum concentration of C4 by ELISA and the hemolytic and antibacterialactivity of CH50 were also evaluated in the current study. The results confirmed that rs132741478: g.2994 A > G in the coding sequence of the ˇ-chain of the bovine C4A gene isrelated to mastitis resistance. This polymorphism may be very important in marker-assistedselections in dairy cattle breeding programs.
Complement component 4 (C4A) is a candidate gene that reflects complement activity. Theprimary role of this gene in the classical and lectin-activation pathways is to provide protectionagainst bacterial pathogens. In the current study, the bovine complement C4A genewas screened for polymorphisms, and the associations of these polymorphisms with thehemolytic activity of the classical pathway (CH50), C4 serum levels, and milk performancetraits were examined. Three novel single-nucleotide polymorphisms (rs 132741478: g.2994A > G, rs 134006517: g.3508 A > G, and rs 137485678: g.3649 G > C) were detected by DNAsequencing and PCR-RFLP in 1182 Chinese Holstein cows. The rs132741478: g.2994 A > Gmutation in exon 10 led to methionine and valine exchange at position 362, whereas rs134006517: g.3508 A >Gandrs137485678: g.3649 G > C were synonymous substitutions.The statistical analyses revealed that cows with rs 132741478: g.2994 A>GAGandrs137485678: g.3649 G > C-CC have significantly lower somatic cell scores (SCS, P < 0.01).Homozygote cows with GAC haplotypes have the lowest SCS, whereas AAG/AAC cows havethe highest. The serum concentration of C4 by ELISA and the hemolytic and antibacterialactivity of CH50 were also evaluated in the current study. The results confirmed that rs132741478: g.2994 A > G in the coding sequence of the ˇ-chain of the bovine C4A gene isrelated to mastitis resistance. This polymorphism may be very important in marker-assistedselections in dairy cattle breeding programs.
